Anti Lamin Antibody What Is It

The full protein is reported to be 664 amino acid residues in length.

Anti lamin antibody what is it.

Anti lamin a c antibody e 1 is a mouse monoclonal igg 1 kappa light chain lamin a c antibody provided at 200 µg ml specific for an epitope mapping between amino acids 2 29 at the n terminus of lamin a c of human origin. Anti lamin a antibody ab26300 at 1 µg ml lane 1. However based on bioassays it appears that the mix of antibodies in this purified polyclonal anti serum bind multiply across the large laminin molecule to an extent that its biological activities are inhibited. Analogies with vertebrate a type lamins.

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. Performed under reducing conditions. Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract. Lamins a and c are alternatively spliced versions of the lmna gene.

Weber k journal of cell science 108 pt 10. This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells. Specific for an epitope mapping between amino acids 580 601 near the c terminus of lamin a of human origin. Anti lamin antibodies are available from several suppliers.

Species predicted to react based on 100 sequence homology. Lamin a c antibodies lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. It is the best among many laminin function blocking preps we have tested. Anti lamin antibody products lamin is a reported alias of the human protein lamin a c encoded by the gene lmna.

These proteins are found in many different cell types in three different forms a b and c. Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx. Lamin a antibody c 3 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml. Expression of drosophila lamin c is developmentally regulated.

Site mapping using a monoclonal antibody. Goat anti rabbit igg h l hrp preadsorbed at 1 5000 dilution developed using the ecl technique. Fisher pa journal of cell science 108 pt 9. Lamin a c antibody detects endogenous levels of total full length lamin a and lamin c 70 kda as well as the small fragment of lamin a and lamin c resulting from cleavage at aspartic acid 230 28 kda.

That is cell receptor binding is markedly hindered. Interphase phosphorylation of the drosophila nuclear lamin. These proteins are found in many different cell types in three different forms a b and c.