Animal Cell Culture Bioreactors

Animal cell culture bioreactors initially employed stirred tanks containing.

Animal cell culture bioreactors.

Morphological and biochemical features of two major mammalian cell groups. The new bioreactor called the see saw bioreactor is ideal for the growth of cells with a sensitive membrane. Requirements for a bioreactor for animal cell culture 1 well controlled environment t ph do nutrients and wastes 2 supply of nutrients 3 gentle mixing avoid shear damage to cells 4 gentle aeration add oxygen slowly to the culture medium but avoid the formation of large bubbles which can damage cells on contact. However despite this sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen especially on a large scale.

The new bioreactor called the see saw bioreactor is ideal for the growth of cells with a sensitive membrane. Microcarriers with adherent cells which were in principle an adaptation of homogeneous culture systems used for. The gas sparging of culture broth causes damage to suspended animal cells. Cell cultures are particularly susceptible to biological contamination.

A bioreactor refers to any manufactured device or system that supports a biologically active environment. Critical contamination points for a bioreactor are the transitions between sterile spaces such as the stirrer ports and gas lines. Bioreactors play a key role in the field of biologics where they are used for the production of recombinant therapeutic proteins by large scale cultivation of animal cells. Animal cell culture control for bioprocess engineering presents the design fabrication and control of a new type of bioreactor meant especially for animal cell line culture.

This article examines the underlying mechanisms involved in bubble associated cell damage and the methods available for controlling such damage. There are several types of bioreactors including stirred tank airlift hollow fiber and rotary cell culture system rccs d. Animal cell culture control for bioprocess engineering presents the design fabrication and control of a new type of bioreactor meant especially for animal cell line culture. It addresses engineering aspects such as mass transfer instrumentation and control ensuring successful design and operation of animal cell bioreactors.

Purpose of this article is to review the current status of bioreactor design for mammalian cell culture. In one case a bioreactor is a vessel in which a chemical process is carried out which involves organisms or biochemically active substances derived from such organisms.